Interrelation between Pseudomonas aeruginosa quinolone signal and the level of immunoglobulins in the blood of patients with lung cancer

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Abstract

Introduction. Researchers in the field of oncology have a significant interest in the role of microorganisms in development of malignant neoplasms.

Aim. To study the levels of 2-heptyl-3-hydroxy-4-quinolone (PQS) and 2-heptyl-4-quinolone (HHQ) produced by Pseudomonas aeruginosa in the blood of patients with lung cancer and to analyze the relation between their changes and changes in the level of immunoglobulins and vascular endothelial growth factor (VEGF) in the blood of patients with lung cancer.

Materials and methods.  PQS and HHQ were quantified in the blood of patients using high performance liquid chromatography. The levels of immunoglobulins G (IgG),  secretory immunoglobulin A (s-IgA),  and VEGF in the blood were determined using ELISA.

Results. Analysis have shown that the level of PQS in the blood of patients with lung cancer is 2-fold higher than in the control group. This change is accompanied by a decrease in the level of immunoglobulins IgG, as well as an increase in the content of s-IgA and growth factor VEGF in the blood.

Conclusion. PQS level in the blood of patients with lung cancer is elevated creating conditions aggravating the course of the main disease and worsening its prognosis.

About the authors

A. V. Shestopalov

Pirogov Russian National Research Medical University, Ministry of Health of Russia; Center of Digital and Translational Biomedicine, Сenter of Molecular Health

Email: fake@neicon.ru
ORCID iD: 0000-0002-1428-7706

1 Ostrovityanova St., Moscow 117997; Bld. 1, 32 Nakhimovskii prospekt, Moscow 117218

Russian Federation

O. I. Кit

National Medical Research Center of Oncology, Ministry of Health of Russia

Email: fake@neicon.ru
ORCID iD: 0000-0003-3061-6108

14th Line, Rostov-on-Don 344037

Russian Federation

V. V. Davydov

Pirogov Russian National Research Medical University, Ministry of Health of Russia

Author for correspondence.
Email: vaddavydov@mail.ru
ORCID iD: 0000-0002-3347-1832

Vadim V. Davydov.

1 Ostrovityanova St., Moscow 117997

Russian Federation

Ya. M. Baizyanova

Pirogov Russian National Research Medical University, Ministry of Health of Russia

Email: fake@neicon.ru

1 Ostrovityanova St., Moscow 117997

Russian Federation

E. Yu. Zlatnik

National Medical Research Center of Oncology, Ministry of Health of Russia

Email: fake@neicon.ru
ORCID iD: 0000-0002-1410-122X

14th Line, Rostov-on-Don 344037

Russian Federation

I. A. Novikova

National Medical Research Center of Oncology, Ministry of Health of Russia

Email: fake@neicon.ru
ORCID iD: 0000-0002-6496-9641

14th Line, Rostov-on-Don 344037

Russian Federation

A. B. Sagakyants

National Medical Research Center of Oncology, Ministry of Health of Russia

Email: fake@neicon.ru
ORCID iD: 0000-0003-0874-5261

14th Line, Rostov-on-Don 344037

Russian Federation

S. A. Appolonova

Center of Digital and Translational Biomedicine, Сenter of Molecular Health

Email: fake@neicon.ru
ORCID iD: 0000-0002-9032-1558

Bld. 1, 32 Nakhimovskii prospekt, Moscow 117218

Russian Federation

N. E. Moskaleva

Center of Digital and Translational Biomedicine, Сenter of Molecular Health

Email: fake@neicon.ru
ORCID iD: 0000-0002-7309-8913

Bld. 1, 32 Nakhimovskii prospekt, Moscow 117218

Russian Federation

S. A. Rumyantsev

Pirogov Russian National Research Medical University, Ministry of Health of Russia; Center of Digital and Translational Biomedicine, Сenter of Molecular Health

Email: fake@neicon.ru

1 Ostrovityanova St., Moscow 117997; Bld. 1, 32 Nakhimovskii prospekt, Moscow 117218

Russian Federation

References

  1. Bagirov N.S., Petukhov I.N., Dmitriev N.V., Grigorevskaia Z.V. Microbiom and cancer: is there interconnection? Literature review. Zlokachestvennye opukholi = Malignant tumors 2018;3(1):56–69. (In Russ.). doi: 10.18027/2224-5057-2018-8-3s1-56-69
  2. Aadra P.B., Matthew R.R., Scott J.B. The role of the microbiome in cancer development and therapy. CA Cancer J Clin 2017;67(4):326–44. doi: 10.3322/caac.21398
  3. Sánchez-Alcoholado L., Ramos-Molina B., Otero A. et al. The Role of the gut microbiome in colorectal cancer development and therapy response. Cancers 2020;12(6):1406. doi: 10.3390/cancers12061406
  4. Teles F.R.F., Alawi F., Castilho R.M., Wang Y. Association or causation? Exploring the oral microbiome and cancer links. J Dent Res 2020;99:1411–24. doi: 10.1177/0022034520945242
  5. Maurice N.M., Bedi B., Sadikot R.T. Pseudomonas aeruginosa biofilms: host response and clinical implications in lung infections. Am J Respir Cell Mol Biol 2018;58(4):428–39. doi: 10.1165/rcmb.2017-0321TR
  6. Riquelme S.A., Liimatta K., Fok Lung T.W. et al. Pseudomonas aeruginosa utilizes host-derived itaconate to redirect its metabolism to promote biofilm formation. Cell Metab 2020;31(6):1091–106. doi: 10.1016/j.cmet.2020.04.017
  7. Garcia-Nuñez M., Marti S., Puig C. et al. Bronchial microbiome, PA biofilm-forming capacity and exacerbation in severe COPD patients colonized by P. aeruginosa. Future Microbiol 2017;12: 379–92. doi: 10.2217/fmb-2016-0127
  8. Biswas A., Mehta H.J., Folch E.E. Chronic obstructive pulmonary disease and lung cancer: inter-relationships. Curr Opin Pulm Med 2018;24(2):152–60. doi: 10.1097/MCP.0000000000000451
  9. Patel B., Priefer R. Impact of chronic obstructive pulmonary disease, lung infection, and/or inhaled corticosteroids use on potential risk of lung cancer. Life Sci 2022;294:120374. doi: 10.1016/j.lfs.2022.120374
  10. Montagut E.J., Marco M.P. Biological and clinical significance of quorum sensing alkylquinolones: current analytical and bioanalytical methods for their quantification. Anal Bioanal Chem 2021;413(18):4599–618. doi: 10.1007/s00216-021-03356-x
  11. Yang L., Yuan T.J., Wan Y. et al. Quorum sensing: a new perspective to reveal the interaction between gut microbiota and host. Future Microbiol 2022;17:293–309. doi: 10.2217/fmb-2021-0217
  12. Liu Y.-C., Hussain F., Negm O. et al. Contribution of the alkylquinolone quorum-sensing system to the interaction of Pseudomonas aeruginosa with bronchial epithelial cells. Front Microbiol 2018;9–11. doi: 10.3389/fmicb.2018.03018
  13. Azam M.W., Khan A.U. Updates on the pathogenicity status of Pseudomonas aeruginosa. Drug Discov Today 2019;24(1):350–9. doi: 10.1016/j.drudis.2018.07.003
  14. Warrier A., Satyamoorthy K., Murali T.S. Quorum-sensing regulation of virulence factors in bacterial biofilm. Future Microbiol 2021;16:1003–21. doi: 10.2217/fmb-2020-0301
  15. Liu C.Y., Xie W.G., Tian J.W., Li J. A comparatively study on inflammatory factors and immune functions of lung cancer and pulmonary ground glass attenuation. Eur Rev Med Pharmacol Sci 2017;21(18):4098–103.
  16. Abolfathi H., Sheikhpour M., Shahraeini S.S. et al. Studies in lung cancer cytokine proteomics: a review. Expert Rev Proteomics 2021;18(1):49–64. doi: 10.1080/14789450.2021.1892491
  17. Rampioni G., Falcone M., Heeb S. et al. Unravelling the genome-wide contributions of specific 2-alkyl-4-quinolones and PqsE to quorum sensing in Pseudomonas aeruginosa. PLoS Pathog 2016;12(11):e1006029. doi: 10.1371/journal.ppat.1006029
  18. Kim K., Kim Y.U., Koh B.H. et al. HHQ and PQS, two Pseudomonas aeruginosa quorum-sensing molecules, down-regulate the innate immune responses through the nuclear factor-kappaB pathway. Immunology 2010;129:578–88. doi: 10.1111/j.1365-2567.2009.03160.x
  19. Hansch G.M., Prior B., Brenner-Weiss G. et al. The Pseudomonas quinolone signal (PQS) stimulates chemotaxis of polymorphonuclear neutrophils. Appl Biomater Funct Mater 2014;12:21–6. doi: 10.5301/jabfm.5000204
  20. Lin J., Cheng J., Wang Y., Shen X. The pseudomonas quinolone signal (PQS): not just for quorum sensing anymore. Front Cell Infect Microbiol 2018;8:230. doi: 10.3389/fcimb.2018.00230
  21. Jing Y., Chen X., Li K. et al. Association of antibiotic treatment with immune-related adverse events in patients with cancer receiving immunotherapy. J Immunother Cancer 2022;10(1):e003779. doi: 10.1136/jitc-2021-003779

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