Expression levels of laminin α5 and the endothelial marker CD146 in tumor tissues from patients with colorectal adenocarcinoma

Cover Page

Cite item

Full Text

Open Access Open Access
Restricted Access Access granted
Restricted Access Subscription or Fee Access

Abstract

Introduction. Colorectal cancer (CRC) is one of the most common malignant neoplasms. Despite significant progress in treatment of this pathology in recent decades, 5-year overall survival of patients with colorectal cancer remains relatively low. The study presents results of comprehensive analysis of laminin α5 (LAMA5) and melanoma cell adhesion molecule (MCAM/CD146) expression levels in primary tumor tissues of patients with this disease. The study is important due to high incidence of CRC and insufficient knowledge of the role of extracellular matrix components in formation of tumor microenvironment.

Aim. To study expression levels of LAMA5 and MCAM (CD146) in tumor tissue of patients with colorectal cancer.

Materials and methods. Analysis of histological sections of human colorectal cancer samples (n = 8) after immunofluorescence staining with antibodies against LAMA5 and CD146 was performed. Qualitative evaluation of expression and colocalization was performed using the ImageJ software with subsequent statistical processing using GraphPad Prism.

Results. Analysis of colocalization per the Manders method showed moderate spatial overlapping of LAMA5 and CD146 signals. Spearman’s rank correlation test showed weak positive correlation between LAMA5 and CD146 expression levels, as well as moderate correlation between their fluorescence intensities. Results of bioinformatics analysis of RNA-seq data from the The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) database confirmed the existence of statistically significant moderate positive correlation between MCAM and LAMA5 gene expression levels.

Conclusion. The obtained data show that in colorectal cancer LAMA5 is located along tumor vascular network and potentially affects its angiogenesis.

About the authors

Arseniy E. Yuzhalin

Sirius University of Science and Technology

Author for correspondence.
Email: yuzhalin.ae@talantiuspeh.ru
ORCID iD: 0000-0001-7454-6653

Scientific Center for Transactional Medicine

 

Russian Federation, 1 Olympic Prospekt, Sirius Federal Territory, Krasnodar Region 354340

K. A. Arsentyev

Sirius University of Science and Technology

Email: yuzhalin.ae@talantiuspeh.ru

Scientific Center for Transactional Medicine

 

Russian Federation, 1 Olympic Prospekt, Sirius Federal Territory, Krasnodar Region 354340

S. A. Savitskii

Sirius University of Science and Technology

Email: yuzhalin.ae@talantiuspeh.ru
ORCID iD: 0009-0007-9487-3863

Scientific Center for Transactional Medicine

 

Russian Federation, 1 Olympic Prospekt, Sirius Federal Territory, Krasnodar Region 354340

References

  1. Saif M.W., Chu E. Biology of colorectal cancer. Cancer J 2010;16(3):196–201. doi: 10.1097/PPO.0b013e3181e076af
  2. Matsuda T., Fujimoto A., Igarashi Y. Colorectal cancer: epidemiology, risk factors, and public health strategies. Digestion 2024;106(2):91–9. doi: 10.1159/000543921
  3. Patel R.K., Rahman S., Schwantes I.R. et al. Updated management of colorectal cancer liver metastases: scientific advances driving modern therapeutic innovations. Cell Mol Gastroenterol Hepatol 2023;16:881–94. doi: 10.1016/j.jcmgh.2023.08.012
  4. Volpi A., D’Elia G., Pannarale O.C. et al. Overexpression of laminin-5 (LN-5) in peritoneal lavage of colorectal cancer patients: preliminary results. G Chir (In Italian) 2011;32(1–2):59–63.
  5. Maltseva D.V., Rodin S.A. Laminins and metastasis of tumors. Molekulyarnaya biologiya = Molecular Biology 2018;52(3):411–34. (In Russ.). doi: 10.7868/S0026898418030059
  6. Zhou B., Zong S., Zhong W. et al. Interaction between laminin-5γ2 and integrin β1 promotes tumor budding of colorectal cancer via activation of Yes-associated proteins. Oncogene 2020;39(7):1527–42. doi: 10.1038/s41388-019-1050-6
  7. Lugli A., Zlobec I., Berger M.D. et al. Tumour budding in solid cancers. Nat Rev Clin Oncol 2021;18(2):101–15. doi: 10.1038/s41571-020-0422-y
  8. Jiang G., Zhang L., Zhu Q. et al. CD146 promotes metastasis and predicts poor prognosis of hepatocellular carcinoma. J Exp Clin Cancer Res 2016;35:38. doi: 10.1186/s13046-016-0313-3
  9. Yang Q., Huang W., Hsu J.C. et al. CD146-targeted nuclear medicine imaging in cancer: state of the art. Eur J Nucl Med Mol Imaging 2023;50(1):5–21. doi: 10.1007/s00259-022-05964-0
  10. Malonne H., Langer I., Kiss R., Atassi G. Mechanisms of tumor angiogenesis and therapeutic implications: angiogenesis inhibitors. Clin Exp Metastasis 1999;17(1):1–14. doi: 10.1023/A:1006605806711
  11. Jiang T., Zhuang J., Duan H. et al. CD146 is a coreceptor for VEGFR-2 in tumor angiogenesis. Blood 2012;120(11):2330–9. doi: 10.1182/blood-2012-01-406108
  12. Wang Z., Xu Q., Zhang N. et al. CD146, from a melanoma cell adhesion molecule to a signaling receptor. Signal Transduct Target Ther 2020;5:148. doi: 10.1038/s41392-020-00259-8
  13. Schindelin J., Arganda-Carreras I., Frise E. et al. Fiji: an open-source platform for biological-image analysis. Nat Methods 2012;9(7):676–82. doi: 10.1038/nmeth.2019
  14. Costes S.V., Daelemans D., Cho E.H. et al. Automatic and quantitative measurement of protein-protein colocalization in live cells. Biophys J 2004;86(6):3993–4003. doi: 10.1529/biophysj.103.038422
  15. Gene Expression Omnibus (GEO). Available at: https://www.ncbi.nlm.nih.gov/geo/
  16. The Cancer Genome Atlas (TCGA). Available at: https://www.cancer.gov/ccg/research/genome-sequencing/tcga
  17. Carloni V., Luong T.V., Rombouts K. Hepatic stellate cells and extracellular matrix in hepatocellular carcinoma: more complicated than ever. Liver Int 2014;34(6):834–5. doi: 10.1111/liv.12541
  18. Liang Y., Zhou X., Xie Q. et al. CD146 interaction with integrin β1 activates LATS1-YAP signaling and induces radiation-resistance in breast cancer cells. Cancer Lett 2022;543:215856. doi: 10.1016/j.canlet.2022.215856
  19. Yousif L.F., Di Russo J., Sorokin L. Laminin isoforms in endothelial and perivascular basement membranes. Cell Adh Migr 2013;7(1): 69–81. doi: 10.4161/cam.22680
  20. Gordon-Weeks A., Lim S.Y., Yuzhalin A. et al. Tumour-derived laminin α5 (LAMA5) promotes colorectal liver metastasis growth, branching angiogenesis and notch pathway inhibition. Cancers (Basel) 2019;11(5):630. doi: 10.3390/cancers11050630
  21. Zhang X., Yan H., Bie L. et al. The role of LAMA5 in breast cancer progression and its potential in immunotherapy. Discov Oncol 2025;16(1):3113. doi: 10.1007/s12672-025-03113-x
  22. Maldonado M.M., Medina J.I., Velazquez L., Dharmawardhane S. Targeting Rac and Cdc42 GEFs in metastatic cancer. Front Cell Dev Biol 2020;8:201. doi: 10.3389/fcell.2020.00201

Supplementary files

Supplementary Files
Action
1. JATS XML
Download

Copyright (c) 2026 ABV-Press

License URL: https://umo.abvpress.ru/jour/about/editorialPolicies
СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).
Регистрационный номер и дата принятия решения о регистрации СМИ: серия ПИ № ФС 77-57560 от  08.04.2014.