Advances in Molecular OncologyAdvances in Molecular OncologyУспехи молекулярной онкологии2313-805X2413-3787Publishing House ABV Press13410.17650/2313-805X-2018-5-1-35-42EXPERIMENTAL RESEARCHЭКСПЕРИМЕНТАЛЬНЫЕ ИССЛЕДОВАНИЯTarget liquid biopsy using “enriched” polymerase chain reaction and DNA melting analysisТаргетная жидкостная биопсия посредством «обогащенной» полимеразной цепной реакции и анализа плавления ДНКhttps://orcid.org/0000-0002-0297-4963BotezatuI. V.БотезатуИ. В.
Russian Federation

24 Kashirskoe Shosse, Moscow 115478

115478 Москва, Каширское шоссе, 24

https://orcid.org/0000-0002-1323-8638PanchukI. O.ПанчукИ. О.
Russian Federation

24 Kashirskoe Shosse, Moscow 115478

115478 Москва, Каширское шоссе, 24

https://orcid.org/0000-0002-6762-9511KolomeytsevaA. A.КоломейцеваА. А.
Russian Federation

24 Kashirskoe Shosse, Moscow 115478

115478 Москва, Каширское шоссе, 24

https://orcid.org/0000-0003-4927-5585FedenkoA. A.ФеденкоА. А.
Russian Federation

24 Kashirskoe Shosse, Moscow 115478

115478 Москва, Каширское шоссе, 24

MazurenkoN. N.МазуренкоН. Н.
Russian Federation

24 Kashirskoe Shosse, Moscow 115478

115478 Москва, Каширское шоссе, 24

TsyganovaI. V.ЦыгановаИ. В.
Russian Federation

24 Kashirskoe Shosse, Moscow 115478

115478 Москва, Каширское шоссе, 24

SusovaO. Yu.СусоваО. Ю.
Russian Federation

24 Kashirskoe Shosse, Moscow 115478

115478 Москва, Каширское шоссе, 24

https://orcid.org/0000-0003-0614-8789KondratovaV. N.КондратоваВ. Н.
Russian Federation

24 Kashirskoe Shosse, Moscow 115478

115478 Москва, Каширское шоссе, 24

https://orcid.org/0000-0002-9837-7273ShelepovV. P.ШелеповВ. П.
Russian Federation

24 Kashirskoe Shosse, Moscow 115478

115478 Москва, Каширское шоссе, 24

https://orcid.org/0000-0002-0190-5069LichtensteinA. V.ЛихтенштейнА. В.
Russian Federation

24 Kashirskoe Shosse, Moscow 115478

115478 Москва, Каширское шоссе, 24

alicht@mail.ruResearch Institute of Carcinogenesis, N.N. Blokhin National Medical Research Center of Oncology, Ministry of Health of RussiaНИИ канцерогенеза ФГБУ «Национальный медицинский исследовательский центр онкологии им. Н.Н. Блохина» Минздрава РоссииResearch Institute of Clinical Oncology, N.N. Blokhin National Medical Research Center of Oncology, Ministry of Health of RussiaНИИ клинической онкологии ФГБУ «Национальный медицинский исследовательский центр онкологии им. Н.Н. Блохина» Минздрава России1501201851354214052018Copyright ©; 2018, Botezatu I.V., Panchuk I.O., Kolomeytseva A.A., Fedenko A.A., Mazurenko N.N., Tsyganova I.V., Susova O.Y., Kondratova V.N., Shelepov V.P., Lichtenstein A.V.Copyright ©; 2018, Ботезату И.В., Панчук И.О., Коломейцева А.А., Феденко А.А., Мазуренко Н.Н., Цыганова И.В., Сусова О.Ю., Кондратова В.Н., Шелепов В.П., Лихтенштейн А.В.2018Botezatu I.V., Panchuk I.O., Kolomeytseva A.A., Fedenko A.A., Mazurenko N.N., Tsyganova I.V., Susova O.Y., Kondratova V.N., Shelepov V.P., Lichtenstein A.V.Ботезату И.В., Панчук И.О., Коломейцева А.А., Феденко А.А., Мазуренко Н.Н., Цыганова И.В., Сусова О.Ю., Кондратова В.Н., Шелепов В.П., Лихтенштейн А.В.https://creativecommons.org/licenses/by/4.0https://umo.abvpress.ru/jour/article/view/134

Objective: to evaluate the possibility of using a highly sensitive method of “enriched” polymerase chain reaction followed by DNA melting analysis (PCR-DMA) for target liquid biopsy of cancer patients.

Materials and methods. The “enriched” PCR-DMA was used for mutation scanning of KRAS (codons 12 and 13) in tumor and blood plasma of 20 patients with colorectal cancer.

Results. Activated oncogene was found in tumor tissue of 16 patients and in blood plasma of 5 patients (confirmed by sequencing). Mutant KRAS alleles were also found in tumor and plasma of another 2 patients, but in very low concentrations that did not allow their validation by Sanger sequencing. Thus, in our study the target liquid biopsy was successful in ~35 % patients. Since the plasma tests were carried out after repeated medical procedures causing mass death of tumor cells, the actual efficiency of this approach may appear significantly higher.

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