Advances in Molecular OncologyAdvances in Molecular OncologyУспехи молекулярной онкологии2313-805X2413-3787Publishing House ABV Press18110.17650/2313-805X-2018-5-4-117-126RESEARCH ARTICLESЭКСПЕРИМЕНТАЛЬНЫЕ СТАТЬИExosomal proteases in colorectal cancerЭкзосомальные протеазы при колоректальном ракеhttps://orcid.org/0000-0003-3698-8455ZambalovaE. A.ЗамбаловаЕ. А.
Russian Federation

5 Kooperativnyy Pereulok, Tomsk 634009

Елена Анатольевна Замбалова.

634009 Томск, пер. Кооперативный, 5

etugutova@mail.ruhttps://orcid.org/0000-0003-2865-7576PatyshevaM. R.ПатышеваМ. Р.
Russian Federation

5 Kooperativnyy Pereulok, Tomsk 634009

634009 Томск, пер. Кооперативный, 5

https://orcid.org/0000-0002-9551-1908DimchaA. A.ДимчаА. А.
Russian Federation

5 Kooperativnyy Pereulok, Tomsk 634009

634009 Томск, пер. Кооперативный, 5

https://orcid.org/0000-0001-7774-943XTamkovichS. N.ТамковичС. Н.
Russian Federation

8 Аkad. Lavrent’eva Rpospekt, Novosibirsk 630090; 2 Pirogova St., Novosibirsk 630090

630090 Новосибирск, пр-т акад. Лаврентьева, 8; 630090 Новосибирск, ул. Пирогова, 2

Grigor’evaA. E.ГригорьеваА. Е.
Russian Federation

8 Аkad. Lavrent’eva Rpospekt, Novosibirsk 630090

630090 Новосибирск, пр-т акад. Лаврентьева, 8

https://orcid.org/0000-0001-9122-3274KolegovaE. S.КолеговаЕ. С.
Russian Federation

5 Kooperativnyy Pereulok, Tomsk 634009

634009 Томск, пер. Кооперативный, 5

https://orcid.org/0000-0003-0907-4615KondakovaI. V.КондаковаИ. В.
Russian Federation

5 Kooperativnyy Pereulok, Tomsk 634009

634009 Томск, пер. Кооперативный, 5

https://orcid.org/0000-0002-4701-0375Afanas’evS. G.АфанасьевС. Г.
Russian Federation

5 Kooperativnyy Pereulok, Tomsk 634009

634009 Томск, пер. Кооперативный, 5

https://orcid.org/0000-0003-4595-4177YunusovaN. V.ЮнусоваН. В.
Russian Federation

5 Kooperativnyy Pereulok, Tomsk 634009; 2 Moskovskiy Tract, Tomsk 634050

634009 Томск, пер. Кооперативный, 5; 634050 Томск, Московский тракт, 2

Cancer Research Institute, Тomsk National Research Medical Center of Russian Academy of SciencesНИИ онкологии, ФГБНУ Томский национальный исследовательский медицинский центр РАНInstitute of Chemical Biology and Fundamental Medicine, Siberian Branch of Russian Academy of SciencesФГБУН Институт химической биологии и фундаментальной медицины Сибирского отделения РАНNovosibirsk National State Research UniversityФГАОУ ВО Новосибирский национальный исследовательский государственный университетSiberian State Medical UniversityФГБОУ ВО «Сибирский государственный медицинский университет» Минздрава России15122018541171260401201904012019Copyright ©; 2018, Zambalova E.A., Patysheva M.R., Dimcha A.A., Tamkovich S.N., Grigor’eva A.E., Kolegova E.S., Kondakova I.V., Afanas’ev S.G., Yunusova N.V.Copyright ©; 2018, Замбалова Е.А., Патышева М.Р., Димча А.А., Тамкович С.Н., Григорьева А.Е., Колегова Е.С., Кондакова И.В., Афанасьев С.Г., Юнусова Н.В.2018Zambalova E.A., Patysheva M.R., Dimcha A.A., Tamkovich S.N., Grigor’eva A.E., Kolegova E.S., Kondakova I.V., Afanas’ev S.G., Yunusova N.V.Замбалова Е.А., Патышева М.Р., Димча А.А., Тамкович С.Н., Григорьева А.Е., Колегова Е.С., Кондакова И.В., Афанасьев С.Г., Юнусова Н.В.https://creativecommons.org/licenses/by/4.0https://umo.abvpress.ru/jour/article/view/181

The objective is to evaluate the level of ADAM10 and ADAM17 (a disintegrin and metalloproteinase) proteases, as well as 20S-proteasomes in blood plasma exosomes of patients with colorectal cancer.

Materials and methods. The study included 60 patients with colorectal cancer (T2–4N0–2M0–1) and 10 control patients. The material for the study was EDTA blood plasma. Exosomes of blood plasma were isolated by ultrafiltration with ultracentrifugation. The level of tetraspanin-associated (ADAM10 and ADAM17) and tetraspanin-non-associated (20S-proteasome) proteases was evaluated by flow cytometry and Western blotting.

Results. A twice negative subpopulation (ADAM10–/ADAM17–) predominated in blood plasma exosomes of colorectal cancer patients and control patients. The level of ADAM10+/ADAM17– exosomes was significantly higher in the exosomes of the plasma of control patients. There were no significant differences between the ADAM10/ADAM17 subpopulations and the 20S-proteasome level, depending on sex, age and tumor grade. A decrease in the ADAM10+/ADAM17– subpopulation was found in patients with metastatic colorectal cancer with hematogenous metastases compared with patients with T2–4N1–2M0 and 20S-proteasome compared to T2–4N0M0. A decrease in ADAM10–/ ADAM17+ exosomes and 20S-proteasomes level was found in exosomes of patients with colorectal cancer with a metabolic syndrome  in comparison with patients without metabolic disorders.

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