Advances in Molecular OncologyAdvances in Molecular OncologyУспехи молекулярной онкологии2313-805X2413-3787Publishing House ABV Press28210.17650/2313-805X-2020-7-3-8-18REVIEW ARTICLESОБЗОРНЫЕ СТАТЬИThe role of extracellular vesicles in the diagnosis of glioblastoma progressionРоль внеклеточных везикул в диагностике прогрессирования глиобластомыhttps://orcid.org/0000-0002-7171-8728RyabovaA. I.РябоваА. И.
Russian Federation

5 Kooperativnyy Pereulok, Tomsk 634009, Russia

Анастасия Игоревна Рябова

Россия, 634009 Томск, переулок Кооперативный, 5

ranigor@mail.ruhttps://orcid.org/0000-0003-0364-0831NovikovV. A.НовиковВ. А.
Russian Federation

5 Kooperativnyy Pereulok, Tomsk 634009, Russia

2 Moskovskiy Trakt, Tomsk 634050, Russia

Россия, 634009 Томск, переулок Кооперативный, 5

Россия, 634050 Томск, Московский тракт, 2

https://orcid.org/0000-0003-4595-4177YunusovaN. V.ЮнусоваН. В.
Russian Federation

5 Kooperativnyy Pereulok, Tomsk 634009, Russia

Россия, 634009 Томск, переулок Кооперативный, 5

https://orcid.org/0000-0003-2060-4840PonomarevaA. A.ПономареваА. А.
Russian Federation

5 Kooperativnyy Pereulok, Tomsk 634009, Russia

Россия, 634009 Томск, переулок Кооперативный, 5

https://orcid.org/0000-0002-5269-736XSpirinaL. V.СпиринаЛ. В.
Russian Federation

5 Kooperativnyy Pereulok, Tomsk 634009, Russia

2 Moskovskiy Trakt, Tomsk 634050, Russia

Россия, 634009 Томск, переулок Кооперативный, 5

Россия, 634050 Томск, Московский тракт, 2

https://orcid.org/0000-0003-1696-427XGribovaO. V.ГрибоваО. В.
Russian Federation

5 Kooperativnyy Pereulok, Tomsk 634009, Russia

Россия, 634009 Томск, переулок Кооперативный, 5

Oncology Research Institute, Tomsk National Research Medical Center, Russian Academy of SciencesНаучно-исследовательский институт онкологии ФГБНУ «Томский национальный исследовательский медицинский центр Российской академии наук»Siberian State Medical University, Ministry of Health of RussiaФГБУ ВО «Сибирский государственный медицинский университет» Минздрава России15092020732311202023112020Copyright ©; 2020, Ryabova A.I., Novikov V.A., Yunusova N.V., Ponomareva A.A., Spirina L.V., Gribova O.V.Copyright ©; 2020, Рябова А.И., Новиков В.А., Юнусова Н.В., Пономарева А.А., Спирина Л.В., Грибова О.В.2020Ryabova A.I., Novikov V.A., Yunusova N.V., Ponomareva A.A., Spirina L.V., Gribova O.V.Рябова А.И., Новиков В.А., Юнусова Н.В., Пономарева А.А., Спирина Л.В., Грибова О.В.https://creativecommons.org/licenses/by/4.0https://umo.abvpress.ru/jour/article/view/282

The article reviews studies highlighting the role of extracellular molecules in non-invasive diagnosis of glioblastoma recurrence. Glioblastoma is the most common malignant tumor of the brain characterized by fatal outcome prognosis. Current treatment of tumor recurrence allows to increase patient survival, improve functional outcome and decrease caregivers» load. The standard method of recurrence diagnosis is neuroimaging which at early stages cannot distinguish between tumor recurrence and post-radiation changes. Currently in oncology, liquid biopsy and marker detection in circulating extracellular vesicles are considered promising approaches allowing to obtain early and differential tumor diagnosis, determine dynamic molecular and genetic status of the tumor, diagnose tumor recurrence at early stages. In this context, the most promising approach to glioblastoma diagnosis is associated with studying of expression of glial fibrillary acidic protein (GFAP), epidermal growth factor receptor (EGFR), its mutant variant EGFRvIII, podoplanin (PDPN) and isocitrate dehydrogenase 1 (IDH1) in extracellular vesicles; for primary glioblastoma diagnosis and early recurrence: studying of microRNA-210, -301a, -222, -123-3p, -21; for control of immunotherapy effectiveness in patients with recurrent forms of glioblastoma after standard treatment: evaluation of СD9+ / GFAP+ / survivin+ exosomes in plasma.

В статье проведен обзор исследований, в которых освещена роль внеклеточных везикул в неинвазивной диагностике рецидива глиобластомы. Глиобластома является самой частой злокачественной опухолью головного мозга у взрослых и характеризуется фатальным прогнозом. Своевременное лечение рецидива опухоли позволяет увеличить выживаемость пациентов, улучшить функциональный исход и снизить нагрузку на ухаживающих. Стандартный метод диагностики рецидива – нейровизуализационные исследования, которые на ранних этапах не позволяют дифференцировать рецидив опухоли от постлучевых изменений. Жидкостная биопсия и выявление маркеров в циркулирующих внеклеточных везикулах считаются перспективным направлением в современной онкологии и позволяют осуществить раннюю и дифференциальную диагностику опухоли, определить молекулярно-генетический статус опухоли в динамике и ответ на лечение, диагностировать рецидив опухоли в ранние сроки. В этом плане для диагностики глиобластом наиболее перспективным является изучение экспрессии глиального фибриллярного кислого белка (GFAP), рецептора эпидермального фактора роста (EGFR), его мутантного варианта EGFRvIII, подопланина (PDPN) и изоцитратдегидрогеназы 1 (IDH1) на внеклеточных везикулах; для первичной диагностики глиобластомы и раннего рецидива – микроРНК- 210, -301a, -222, -124-3p, -21; для контроля эффективности иммунотерапии больных с рецидивными формами глиобластом после стандартной терапии – оценка СD9+ / GFAP+ / survivin+ экзосом плазмы крови.

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