Advances in Molecular OncologyAdvances in Molecular OncologyУспехи молекулярной онкологии2313-805X2413-3787Publishing House ABV Press3010.17650/2313-805X.2014.1.2.74-78АDVERTISING PUBLICATIONНА ПРАВАХ РЕКЛАМЫAmniocar as a proliferative medium for mesenchymal cellsАмниокар – пролиферативная среда для мезенхимальных клетокChestkovV. V.ЧестковВ. В.
Russian Federation
chestkov@med-gen.ruTabakovV. Yu.ТабаковВ. Ю.
Russian Federation
ShchepkinaYu. V.ЩепкинаЮ. В.
Russian Federation
Medical and Genetic Reseach Center, Russian Academy of Medical Sciences, Russia, 115478, Moscow, Zamoskvorechiye St., 1ФГБУ «Медико-генетический научный центр» РАМН, Россия, 115478, Москва, ул. Москворечье, 1NPP "PanEco" LLC, Russa, 115522, Moscow, p/o box 119ООО НПП «ПанЭко», Россия, 115522, Москва, а / я 119150520141274780306201503062015Copyright ©; 2014, Chestkov V.V., Tabakov V.Y., Shchepkina Y.V.Copyright ©; 2014, Честков В.В., Табаков В.Ю., Щепкина Ю.В.2014Chestkov V.V., Tabakov V.Y., Shchepkina Y.V.Честков В.В., Табаков В.Ю., Щепкина Ю.В.https://creativecommons.org/licenses/by/4.0https://umo.abvpress.ru/jour/article/view/30

Objectives. To develop the Amniocar nutrient medium that contains fetal calf serum (FCS) and growth factors cocktail for mass cultivation of human fibroblasts. To study proliferative activity of the medium on cultures of HUVEC cells of mesenchymal origin and mesenchymal stromal cells, as well as on cell culture of human amniotic fluid.

Materials and methods. Determination of the rate of accumulation of the cellular mass and cell morphology in the course of cultivation of cells of various histogenesis in the Amniocar medium and nutrient medium that contains 10 % of FCS.

Results. It has been demonstrated that the Amniocar medium is prevalent as compared to the standard DMEM medium with 10 % of FCS by 2 to 5 times for cultivation of skin fibroblasts, HUVEC, and mesenchymal stem cells. The Amniocar medium increased the quantity of endothelial cells that enter mitosis and maintained the culture of HUVEC cells with prolonged passaging in vitro. Clonal cultivation of human amniotic fluid cells in the Amniocar medium secured development of colonies of both fibroblast and epithelial type.

Conclusions. Proliferative Amniocar medium is efficient for mass cultivation of various cells of mesenchymal origin and can be used for diagnostic purposes in medical genetics, oncology, etc.

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