Advances in Molecular OncologyAdvances in Molecular OncologyУспехи молекулярной онкологии2313-805X2413-3787Publishing House ABV Press5510.17650/2313-805X.2016.3.1.53-60RESEARCH ARTICLESЭКСПЕРИМЕНТАЛЬНЫЕ СТАТЬИTissue-specificity of proteoglycans expression in different cancersТканеспецифичность экспрессии протеогликанов в различных типах опухолей человекаSuhovskihA. V.СуховскихА. В.
Russian Federation

2/12 Timakova St., Novosibirsk, 630117;

2 Pirogova St., Novosibirsk, 630090

630117, Новосибирск, ул. Тимакова, 2/12;

630090, Новосибирск, ул. Пирогова, 2

anastasia-suhovskih@mail.ruGrigor’evaE. V.ГригорьеваЭ. В.
Russian Federation

2/12 Timakova St., Novosibirsk, 630117;

2 Pirogova St., Novosibirsk, 630090

630117, Новосибирск, ул. Тимакова, 2/12;

630090, Новосибирск, ул. Пирогова, 2

Institute of Molecular Biology and BiophysicsФГБНУ «Научно-исследовательский институт молекулярной биологии и биофизики»Novosibirsk State UniversityФГАОУ ВО «Новосибирский национальный исследовательский государственный университет»150120163153602504201625042016Copyright ©; 2016, Suhovskih A.V., Grigor’eva E.V.Copyright ©; 2016, Суховских А.В., Григорьева Э.В.2016Suhovskih A.V., Grigor’eva E.V.Суховских А.В., Григорьева Э.В.https://creativecommons.org/licenses/by/4.0https://umo.abvpress.ru/jour/article/view/55

Background. Proteoglycans (PGs) are complex glycosylated molecules playing an important role in cell-cell and cell-matrix interactions and signaling. Expression of PGs and their expression pattern change considerably during malignant transformation of mammalian cells and tissues.

Objective. The aim of our work was to investigate tissue-specificity of main PGs expression (glypican-1, perlecan, syndecan-1, aggrecan, versican, CSPG4/NG2, brevican, decorin, lumican) in normal cells (fibroblasts and normal epithelial prostate cells PNT2) and in different human cancer cell lines (prostate, breast, lung, brain, kidney). Expression patterns of main PGs were determined in these cells using reverse transcription polymerase chain reaction analysis and immunocytochemical staining.

Results. It was shown that fibroblasts actively expressed PGs, and PNT2 cells had lower (5–6-fold) expression levels of a limited set of PG. In different cancer cell lines, overall transcriptional activities of PGs varied up to 10-fold, although their expression patterns had tissue-specific properties (for example, expression of syndecan-1 is more specific for prostate cancer cells, while perlecan is typical for lung cancer cell lines).

Conclusions. Along with this, variability of the PG expression patterns in cell lines of the same tissue of origin was shown, suggesting a possible contribution of the variable PGs expression to intratumoural heterogeneity of cancer cells and their potential as perspective biomarker (s) for personalised cancer diagnostics.

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