Advances in Molecular OncologyAdvances in Molecular OncologyУспехи молекулярной онкологии2313-805X2413-3787Publishing House ABV Press6210.17650/2313-805X.2016.3.2.50-59RESEARCH ARTICLESЭКСПЕРИМЕНТАЛЬНЫЕ СТАТЬИMolecular determinants of transforming growth factor beta-1 action on human glioblastoma cellsМолекулярные детерминанты действия трансформирующего фактора роста бета-1 на клетки глиобластомы человекаShevchenkoV. E.ШевченкоВ. Е.
Russian Federation
vshev2015@yandex.ruKovalevS. V.КовалевС. В.
Russian Federation
ArnotskayaN. E.АрноцкаяН. Е.
Russian Federation
NikiforovaZ. N.НикифороваЗ. Н.
Russian Federation
KudryavtsevI. A.КудрявцевИ. А.
Russian Federation
SavchenkoE. A.СавченкоЕ. А.
Russian Federation
BryukhovetskiyI. S.БрюховецкийИ. С.
Russian Federation
Research Institute of Carcinogenesis, N.N. Blokhin Russian Cancer Research Center, Ministry of Health of RussiaНИИ канцерогенеза ФГБУ «РОНЦ им. Н. Н. Блохина» Минздрава России24 Kashirskoe Shosse, Moscow, 115478, RussiaРоссия, 115478, Москва, Каширское шоссе, 24Department of Chemistry, M. V. Lomonosov Moscow State Universityхимический факультет ФГБОУ ВО «Московский государственный университет им. М. В. Ломоносова»GSP-1, Build. 3, 1 Leninskie Gory, Moscow, 119991, RussiaРоссия, 119991, Москва, Ленинские горы, 1, стр. 3, ГСП-1Institute of Gene Biology, Russian Academy of SciencesФГБУН «Институт биологии гена» РАН34/5 Vavilova St., Moscow, 119334, RussiaРоссия, 119334, Москва, ул. Вавилова, 34/5Biomedicine School, Far Eastern Federal UniversityШкола биомедицины ФГАОУ ВПО «Дальневосточный федеральный университет»8 Sukhanova St., Vladivostok, 690091, RussiaРоссия, 690091, Владивосток, ул. Суханова, 8A. V. Zhirmunsky Institute of Sea Biology, Far Eastern Brach, Russian Academy of SciencesФГБУН «Институт биологии моря им. А. В. Жирмунского» Дальневосточного отделения РАН17 Pal’chevskogo St., Vladivostok, 690059, RussiaРоссия, 690059, Владивосток, ул. Пальчевского, 17150520163250590706201607062016Copyright ©; 2016, Shevchenko V.E., Kovalev S.V., Arnotskaya N.E., Nikiforova Z.N., Kudryavtsev I.A., Savchenko E.A., Bryukhovetskiy I.S.Copyright ©; 2016, Шевченко В.Е., Ковалев С.В., Арноцкая Н.Е., Никифорова З.Н., Кудрявцев И.А., Савченко Е.А., Брюховецкий И.С.2016Shevchenko V.E., Kovalev S.V., Arnotskaya N.E., Nikiforova Z.N., Kudryavtsev I.A., Savchenko E.A., Bryukhovetskiy I.S.Шевченко В.Е., Ковалев С.В., Арноцкая Н.Е., Никифорова З.Н., Кудрявцев И.А., Савченко Е.А., Брюховецкий И.С.https://creativecommons.org/licenses/by/4.0https://umo.abvpress.ru/jour/article/view/62

Background. Increased expression of transforming growth factor beta-1 (TGF-β1) in malignant brain tumors promotes cancer cells survival enhancing their growth, migration, invasion, angiogenesis, immune system suppression.

Objective is to study molecular mechanisms of TGF-β1 action on U87 human glioblastoma cells by means of proteomic high-resolution massspectrometry.

Results. We have identified intracell signal pathways responsible for TGF-β1 involvement in malignant gliomas oncogenesis including differential expressed proteins of tight cell junctions, focal adhesion, histone deacetylases, heat shock, S100 family.

Conclusions. Important patterns are determined that could be used for the development of new approaches for detection of glioblastoma metastasis candidate markers and potential therapy targets of this decease.

Введение. Повышенная экспрессия трансформирующего фактора роста бета-1 (transforming growth factor beta1 , TGF-β1) в злокачественных опухолях головного мозга способствует выживанию опухолевых клеток, увеличивая их рост, миграцию, инвазию, ангиогенез, супрессию иммунной системы.

Цель работы – методом протеомной масс-спектрометрии высокого разрешения изучить молекулярные механизмы действия TGF-β1 на клетки U87 глиобластомы человека.

Результаты. Идентифицированы внутриклеточные сигнальные пути, ответственные за участие TGF-β1 в онкогенезе злокачественных глиом и включающие дифференциально экспрессированные белки плотных межклеточных контактов, фокальной адгезии, деацелаз гистонов, теплового шока, семейства S100.

Заключение. Установлены важные закономерности, которые могут быть использованы при разработке новых подходов для обнаружения кандидатных маркеров метастазирования глиобластомы и потенциальных мишеней для терапии этого заболевания.

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