Email this article Target liquid biopsy using “enriched” polymerase chain reaction and DNA melting analysis
- Authors: Botezatu I.V.1, Panchuk I.O.1, Kolomeytseva A.A.2, Fedenko A.A.2, Mazurenko N.N.1, Tsyganova I.V.1, Susova O.Y.1, Kondratova V.N.1, Shelepov V.P.1, Lichtenstein A.V.1
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Affiliations:
- Research Institute of Carcinogenesis, N.N. Blokhin National Medical Research Center of Oncology, Ministry of Health of Russia
- Research Institute of Clinical Oncology, N.N. Blokhin National Medical Research Center of Oncology, Ministry of Health of Russia
- Issue: Vol 5, No 1 (2018)
- Pages: 35-42
- Section: EXPERIMENTAL RESEARCH
- Published: 14.05.2018
- URL: https://umo.abvpress.ru/jour/article/view/134
- DOI: https://doi.org/10.17650/2313-805X-2018-5-1-35-42
- ID: 134
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Abstract
Objective: to evaluate the possibility of using a highly sensitive method of “enriched” polymerase chain reaction followed by DNA melting analysis (PCR-DMA) for target liquid biopsy of cancer patients.
Materials and methods. The “enriched” PCR-DMA was used for mutation scanning of KRAS (codons 12 and 13) in tumor and blood plasma of 20 patients with colorectal cancer.
Results. Activated oncogene was found in tumor tissue of 16 patients and in blood plasma of 5 patients (confirmed by sequencing). Mutant KRAS alleles were also found in tumor and plasma of another 2 patients, but in very low concentrations that did not allow their validation by Sanger sequencing. Thus, in our study the target liquid biopsy was successful in ~35 % patients. Since the plasma tests were carried out after repeated medical procedures causing mass death of tumor cells, the actual efficiency of this approach may appear significantly higher.
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About the authors
I. V. Botezatu
Research Institute of Carcinogenesis, N.N. Blokhin National Medical Research Center of Oncology, Ministry of Health of Russia
Email: fake@neicon.ru
ORCID iD: 0000-0002-0297-4963
24 Kashirskoe Shosse, Moscow 115478 Russian Federation
I. O. Panchuk
Research Institute of Carcinogenesis, N.N. Blokhin National Medical Research Center of Oncology, Ministry of Health of Russia
Email: fake@neicon.ru
ORCID iD: 0000-0002-1323-8638
24 Kashirskoe Shosse, Moscow 115478 Russian Federation
A. A. Kolomeytseva
Research Institute of Clinical Oncology, N.N. Blokhin National Medical Research Center of Oncology, Ministry of Health of Russia
Email: fake@neicon.ru
ORCID iD: 0000-0002-6762-9511
24 Kashirskoe Shosse, Moscow 115478 Russian Federation
A. A. Fedenko
Research Institute of Clinical Oncology, N.N. Blokhin National Medical Research Center of Oncology, Ministry of Health of Russia
Email: fake@neicon.ru
ORCID iD: 0000-0003-4927-5585
24 Kashirskoe Shosse, Moscow 115478 Russian Federation
N. N. Mazurenko
Research Institute of Carcinogenesis, N.N. Blokhin National Medical Research Center of Oncology, Ministry of Health of Russia
Email: fake@neicon.ru
24 Kashirskoe Shosse, Moscow 115478 Russian Federation
I. V. Tsyganova
Research Institute of Carcinogenesis, N.N. Blokhin National Medical Research Center of Oncology, Ministry of Health of Russia
Email: fake@neicon.ru
24 Kashirskoe Shosse, Moscow 115478 Russian Federation
O. Yu. Susova
Research Institute of Carcinogenesis, N.N. Blokhin National Medical Research Center of Oncology, Ministry of Health of Russia
Email: fake@neicon.ru
24 Kashirskoe Shosse, Moscow 115478 Russian Federation
V. N. Kondratova
Research Institute of Carcinogenesis, N.N. Blokhin National Medical Research Center of Oncology, Ministry of Health of Russia
Email: fake@neicon.ru
ORCID iD: 0000-0003-0614-8789
24 Kashirskoe Shosse, Moscow 115478 Russian Federation
V. P. Shelepov
Research Institute of Carcinogenesis, N.N. Blokhin National Medical Research Center of Oncology, Ministry of Health of Russia
Email: fake@neicon.ru
ORCID iD: 0000-0002-9837-7273
24 Kashirskoe Shosse, Moscow 115478 Russian Federation
A. V. Lichtenstein
Research Institute of Carcinogenesis, N.N. Blokhin National Medical Research Center of Oncology, Ministry of Health of Russia
Author for correspondence.
Email: alicht@mail.ru
ORCID iD: 0000-0002-0190-5069
24 Kashirskoe Shosse, Moscow 115478 Russian Federation
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