Exosomal proteins as potential markers of multiple myeloma diagnostics

V. E. Shevchenko; Шевченко В. Е.; A. S. Bryukhovetskiy; Брюховецкий А. С.; M. V. Filatov; Филатов М. В.; V. S. Burdakov; Бурдаков В. С.; Z. N. Nikiforova; Никифорова З. Н.; I. S. Bryukhovetskiy; Брюховецкий И. С.; Yu. D. Vasilets; Василец Ю. Д.; T. I. Kushnir; Кушнир Т. И.; N. E. Arnotskaya; Арноцкая Н. Е.

doi:10.17650/2313-805X-2018-5-1-60-69

Exosomal proteins as potential markers of multiple myeloma diagnostics

Authors: Shevchenko V.E.¹, Bryukhovetskiy A.S.², Filatov M.V.³, Burdakov V.S.³, Nikiforova Z.N.⁴, Bryukhovetskiy I.S.⁵^,6, Vasilets Y.D.⁷, Kushnir T.I.⁷, Arnotskaya N.E.¹
Affiliations:
1. Research Institute of Carcinogenesis, N.N. Blokhin National Medical Research Center of Oncology, Ministry of Health of Russia
2. NeuroVita Clinic of Interventional and Restorative Neurology and Therapy
3. National Research Center “Kurchatov Institute”, B.P. Konstantinov Petersburg Nuclear Physics Institute
4. All-Russian State Center for Quality and Standardization of Veterinary Drugs and Feed
5. School of Biomedicine, Far Eastern Federal University
6. National Scientific Center of Marine Biology, Far Eastern Branch of the Russian Academy of Sciences
7. K.I. Skryabin Moscow State Academy of Veterinary Medicine and Biotechnology
Issue: Vol 5, No 1 (2018)
Pages: 60-69
Section: EXPERIMENTAL RESEARCH
Published: 14.05.2018
URL: https://umo.abvpress.ru/jour/article/view/137
DOI: https://doi.org/10.17650/2313-805X-2018-5-1-60-69
ID: 137

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Abstract

Background. Multiple myeloma (MM) is a hematologic malignancy of plasma cells. The microenvironment plays a key role in MM cell survival and drug resistance through release of soluble factors, expression of adhesion molecules and release of exosomes (EXs). The role that EXs, released by MM cells have in cell-to-cell communication and signaling in the bone marrow is currently unknown. EXs as a source of markers for MM diagnostics are also not studied.

Objective: to use proteomic profiling of EXs as a tool to identify circulating tumor associated markers in MM patients.

Results. The proteome composition of EXs obtained from plasma of patients with MM and multiple sclerosis was studied for the first time. nano-HPLC–MS/MS analysis identified a total of 332 proteins in the EXs of both groups of patients and determined the proximity of their qualitative composition. For the first time, 12 differentially expressed proteins were detected, the levels of which were significantly increased in EXs from patients with MM. This allowed us to consider them as potential markers of the disease.

Conclusion. Proteomic analysis of EXs obtained from plasma of patients with MM is an important method for finding disease markers.

Keywords

exosome, blood plasma, proteome, mass-spectrometry, мultiple myeloma, multiple sclerosis

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Exosomal proteins as potential markers of multiple myeloma diagnostics

Full Text

Abstract

Keywords

About the authors

References

Supplementary files