Email this article Immunohistochemical analysis of prame expression as a prognostic factor in uveal melanoma patients
- Authors: Saakyan S.V.1, Tsygankov A.Y.1, Amiryan A.G.1, Khlgatyan M.R.1, Khochenkov D.A.2, Misyurin V.A.2
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Affiliations:
- Helmholtz National Medical Research Center of Eye Diseases, Ministry of Health of Russia
- N.N. Blokhin National Medical Research Center of Oncology, Ministry of Health of Russia
- Issue: Vol 8, No 4 (2021)
- Pages: 61-66
- Section: RESEARCH ARTICLES
- Published: 18.12.2021
- URL: https://umo.abvpress.ru/jour/article/view/390
- DOI: https://doi.org/10.17650/2313-805X-2021-8-4-61-66
- ID: 390
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Abstract
The study objective is to analyze the prognostic significance of PRAME protein expression in patients with uveal melanoma using immunohistochemical assay.
Materials and methods. A total of 30 patients with uveal melanoma were examined and treated. The average age of patients at the time of treatment was 51.3 ± 11.8 years. In all cases, enucleation was performed according to the indications. A routine clinical-morphological, molecular-genetic, and immunohistochemical assay study was performed (n = 29). The immunohistochemical study was performed using antibodies to PRAME (clone 6H8, dilution 1: 50). The median follow-up period was 86.3 ± 2.9 months.
Results. Of the 29 samples studied, staining was determined in 16, which was 55.2 %. Weak intensity of 1+ staining (from 10 to 20 % of tumor cells) was detected in 7 uveal melanoma samples, medium intensity of 2+ (from 10 to 20 % of tumor cells) – also in 7, and strong intensity of 3+ (30 % of tumor cells) – in 2 tumor samples. When assessing the seven-year survival, the cumulative survival rate in the group without PRAME expression was 0.857, while in the group with PRAME expression it was significantly lower and amounted to 0.357 (p = 0.0001). The PRAME protein expression was significantly correlated with the epithelioid cell type of the tumor (p = 0.041) and with the total and partial monosomy of chromosome 3 (p = 0.013).
Conclusion. This paper presents the world’s first study of the prognostic significance of PRAME protein expression by immunohistochemical analysis in patients with uveal melanoma. A significant association of PRAME-positive patients with an unfavorable vital prognosis was shown.
Keywords
About the authors
S. V. Saakyan
Helmholtz National Medical Research Center of Eye Diseases, Ministry of Health of Russia
Email: fake@neicon.ru
ORCID iD: 0000-0001-8591-428X
14/19, Sadovaya-Chernogryazskaya St., Moscow 105062
Russian FederationA. Yu. Tsygankov
Helmholtz National Medical Research Center of Eye Diseases, Ministry of Health of Russia
Author for correspondence.
Email: alextsygankov1986@yandex.ru
ORCID iD: 0000-0001-9475-3545
Alexander Yuryevich Tsygankov
14/19, Sadovaya-Chernogryazskaya St., Moscow 105062
Russian FederationA. G. Amiryan
Helmholtz National Medical Research Center of Eye Diseases, Ministry of Health of Russia
Email: fake@neicon.ru
ORCID iD: 0000-0002-9284-4838
14/19, Sadovaya-Chernogryazskaya St., Moscow 105062
Russian FederationM. R. Khlgatyan
Helmholtz National Medical Research Center of Eye Diseases, Ministry of Health of Russia
Email: fake@neicon.ru
ORCID iD: 0000-0001-7266-770X
14/19, Sadovaya-Chernogryazskaya St., Moscow 105062
Russian FederationD. A. Khochenkov
N.N. Blokhin National Medical Research Center of Oncology, Ministry of Health of Russia
Email: fake@neicon.ru
ORCID iD: 0000-0002-5694-3492
23 Kashirskoe Shosse, Moscow 115478
Russian FederationV. A. Misyurin
N.N. Blokhin National Medical Research Center of Oncology, Ministry of Health of Russia
Email: fake@neicon.ru
ORCID iD: 0000-0002-0762-5631
23 Kashirskoe Shosse, Moscow 115478
Russian FederationReferences
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