STUDY OF THE RELATIONSHIP BETWEEN CIRCADIAN RHYTHMS AND DRUG RESISTANCE OF BREAST TUMOR CELL LINES

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Abstract

10 % of genome mRNA expression is rhythmic and these 24-hrs rhythms are under control of the circadian clock. Epidemiologic studies have revealed a clear link between the disruption of circadian rhythms and cancer development in humans. Growing evidence shows that circadian disruption is associated with development of malignant tumors, including breast cancer. Aim of this study was to investigate: the expression of circadian clock genes in human mammary epithelial cell line MCF10A and breast cancer cell lines MCF-7, ZR-75-1, BT-474 and if the multidrug resistance phenotype of cancer cells is associated with changes in circadian clock genes expression. We have found that Per1 expression significantly reduced in cancer cells. No correlation was detected between the expression of circadian clock genes and cancer breast cell lines drug resistance. Interestingly, the expression of Bmal1, Per1 and Cry1 were increased in multi-drug resistant MCF-7_D cells compare with the parent cells MCF-7 cells, however, if these changes in the expression contribute to the drug-resistance or not is not clear. These results argue for further study.

About the authors

A. M. Оgloblina

Research Institute of Carcinogenesis, N.N. Blokhin Russian Cancer Research Center, Ministry of Health of Russia, Moscow

Email: susovaolga@gmail.com
Russian Federation

E. Yu. Rybalkina

Research Institute of Carcinogenesis, N.N. Blokhin Russian Cancer Research Center, Ministry of Health of Russia, Moscow

Email: susovaolga@gmail.com
Russian Federation

K. I. Кirsanov

Research Institute of Carcinogenesis, N.N. Blokhin Russian Cancer Research Center, Ministry of Health of Russia, Moscow

Email: susovaolga@gmail.com
Russian Federation

N. I. Moiseeva

Research Institute of Carcinogenesis, N.N. Blokhin Russian Cancer Research Center, Ministry of Health of Russia, Moscow

Email: susovaolga@gmail.com
Russian Federation

R. V. Кondratov

Cleveland State University, Cleveland

Email: susovaolga@gmail.com
United States

O. Yu. Susova

Research Institute of Carcinogenesis, N.N. Blokhin Russian Cancer Research Center, Ministry of Health of Russia, Moscow

Author for correspondence.
Email: susovaolga@gmail.com
Сусова Ольга Юрьевна Russian Federation

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